Electrochemical Detection Of Fluoroquinolone Antibiotics In ...
Software (GraphPad Software, San Diego, CA, USA). The magnetic separation during the washing steps was performed using a magnetic separator Dynal MPC-S (Dynal Biotech ASA, Norway) or 96-Well Plate Separation Rack (Cortex Biochem, CA, USA). The pH and conductivity of all buffers ... Read Full Source
4-FB Magnetic Beads - TriLink BioTech
Standard commercially available magnetic racks suitable for washing and handling paramagnetic microspheres. On the left is a 2-tube magnetic rack and on the right is a 6-tube magnetic rack (MPC-S) both manufactured by Dynal, Inc. C. Materials Required PepLinkTM peptide (0.5 mg solid) (HyNic-activated peptide @ NH 2 terminus) ... Doc Viewer
Maximizing Capture Efficiency And Specificity Of Magnetic ...
Bacterium complexes, samples were placed in a magnetic separator rack (Dynal MPC-M) for 10 min. The sample supernatant was carefully aspirated off with a sterile Pasteur pipette and was discarded. Beads were washed twice with 1 ml ... Document Viewer
Comparison Of The Utility Of Five Commercial Kits For ...
Lysis buffer containing magnetic beads (Dynabeads) were dispensed to 1.5-ml tubes. The tubes were placed in a Dynal MPC-S magnetic rack to allow the Dynabeads to move to one side of the tube. The supernatant (lysis buffer without the Dynabeats) was transferred to a tube containing pellet formed after lysis of blood cells. After ... Fetch This Document
Chapter 11
Separating on a magnetic rack for 2 min between washes. 9. Resuspend coated beads in 500 l storage buffer, which is twice the original volume of uncoated beads used. This Þ nal volume is sufÞ cient for 50 IMS reactions when 10 l of coated In the absence of a Dynal BeadRetrieverª, IMS may ... Fetch Doc
Development Of A Fluorescent Enzyme-Linked DNA Aptamer ...
System based onour previouscapture aptamer-magnetic bead (MB) and fluorescent reporter aptamer sandwich assay methods for Campylobacter [8] and Leishmania parasites [9]. We surmised that these former technologies could be adapted to the use of surface swabs or “spongicles” which can be squeezed to yield 2 ml of buffer which has interacted ... Return Doc
How To Use Dynabeads® For Immunoprecipitation - YouTube
One of the beauties of magnetic handling is that you can work with concentrated samples throughout the procedure, and you won't need to pre-treat or dilute the sample. You only need 1-10 ... View Video
Major Histocompatibility Complex–mismatched Allogeneic Bone ...
With Dynal Beads (Dynal Biotech, Oslo, Norway). Briefly, donor spleen and lymph node cells were incubated with anti-CD4–conjugated Dynal magnetic beads for 20 minutes. The cells binding to Dynal beads were then precipitated on a magnetic rack, and the cells in suspension were discarded. ... Fetch Full Source
Horses And Other Equids Actinomyces Denticolens Colonisation ...
Afterwards, the tubes were mounted on a magnetic rack (Dynal MPCTM-S Life Technologies, USA) and were shaken slowly for one hour in order to allow the immu-noreaction to occur at room temperature. Then 20 μlof magnetic-beads-bound sheep anti-rabbit IgG was added into the tubes, and the tubes were again mounted on ... Fetch Document
Dynabeads Anti-Salmonella - Techmicrobio.eu
Each sample into a sample rack. 2. Resuspend Dynabeads anti-Salmonella by vor- Invert the Dynal MPC-S rack five times to mix sample and beads. Incubate at room tempera- Dynal Magnetic particle 120.20D 1 Unit ... Fetch Doc
Amperometric Detection Of Staphylococcal Enterotoxin B In ...
Separator (Dynal MPC®-S, Dynal MPC magnetic rack, and MBs were allowed to separate for 2 min before the milk was removed and MBs were washed one time with 150 µl PBST buffer. A solution (1 µg/ml) of biotinylated anti-SEB polyclonal antibody in PBSTB (100 ... Fetch This Document
Chapter 25 Affinity Maturation By Chain Shuffling And Site ...
– Streptavidin-magnetic beads (Dynal) – Magnetic rack (Dynal MPC-E) – PD10 colums (GE Healthcare) – CM5 sensor chip (BIAcore) – SOC (recipe: To 950 mL deionized H 2O, add 20 g bacto-tryptone, 5 g yeast extract,and 0.5gNaCl. Add10mLofa250mMKClsolution.AdjustthepH to 7.0 with 5 N NaOH, and make up the volume of the solution to 1 L with ... Access Full Source
I C A L EE N G Ine Ring M E D E Journal Of O D I F Ac ...
Dynal MPC-S magnetic rack. Five hundred µg of various 5’ biotinylated reporter aptamers specific for each assay target were added to 50 µl of each type of colored streptavidin-QD in 1 ml of sterile PBS and gently mixed at room temperature for 1 h. Aptamer-5’-biotin-streptavidin-QD conjugates were then purified in the void ... Access Doc
Growth Factor Stimulation Of Cryopreserved CD34 + Bone Marrow ...
5% BCS. Polystyrene beads (150 ml) (Dynal) covered with sheep antimouse antibodies were added, and the cells were incubated for 45 min at 4°C on a rotating rack. Next, the cells were trans- ferred to five ml polystyrene plastic tubes and collected for two min using a magnetic rack (Dynal). The magnetic separation was repeated three times. ... Access This Document
Dynabeads Anti-Legionella - Thermo Fisher Scientific
Dynabeads anti-Legionella are uniform, superpara-magnetic, polystyrene microscopic beads with antibodies against Legionella covalently bound to the surface. The beads are supplied in a suspensi- Invert the Dynal MPC-S rack five times. ... Retrieve Doc
Affinity Maturation By Chain Shuffling And Site Directed ...
- Streptavidin-magnetic beads (Dynal) - Magnetic rack (Dynal MPC-E) - PDlO colums (Pharmacia) - CM5 sensor chip (BIAcore) - soc (recipe: to 950 ml deionized H20 add: 20 g bacto-tryptone, 5 g yeast extract, 0.5 g NaCl. Add 10 ml of a 250 mM KCl solution. Adjust the pH to 7.0 with 5 M NaOH and adjust the volume of the solution to 11 ... View Document
Cat. No. 120.21D Rev. No. 003 Dynal MPC™-L
The Dynal MPC-L Magnetic Particle Concentrator contains a plastic housing and a removable mag-net slide incorporating high-energy neodymium magnets. The magnet is designed to hold six separate FACS or Vacutainer tubes (10–16 mm in diameter) with ... Doc Viewer
4FB Magnetic Beads - TriLink BioTech
Standard commercially available magnetic racks suitable for washing and handling paramagnetic microspheres. On the left is a 2-tube magnetic rack and on the right is a 6-tube magnetic rack (MPC-S) both manufactured by Dynal, Inc. ... Retrieve Doc
Chapter 11 Improved Detection Of Mycobacterium Bovis In ...
Manual IMS using magnetic rack In the absence of a Dynal BeadRetriever™, IMS may be performed manually ( see Note 8 ) using 1.5 ml microcentrifuge tubes and a suitable magnetic rack ( see Note 9 ). ... Access Full Source
Magnetic Separation Using Dynabeads® - YouTube
Http://www.lifetechnologies.com/dynab Magnetic handling allows you to simplify the separation of cells, proteins, nucleic acids and other biological materials ... View Video
Transforming Single DNA Molecules Into Fluorescent magnetic ...
Magnetic particles for detection and enumeration were purchased from Dynal Biotech (no. 650.01, Lake Success, NY). Beads were washed once with 1 PCR tubes in a rack placed at the center of the magnetic stirrer. Step 3: PCR Cycling. ... Access This Document
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